Paper A25

Potential Application of Fluorescence Spectroscopy for
Authentication of Bee Propolis: A Preliminary Study

Slamet Widodo*,1, Usman Ahmad1, Tetsuhito Suzuki2, Yuichi Ogawa2, Naoshi Kondo2
1Department of Mechanical and Biosystem Engineering, Bogor Agricultural University, Indonesia
2Graduate School of Agriculture, Kyoto University, Japan
Email: slamet_ae39@apps.ipb.ac.id, slamet.ae39@gmail.com*

Abstract

Propolis as one of honey bee products has been well known for its medicinal properties
such as anti-bacterial, anti-viral, anti-fungal, anti-oxidant, anti-inflammatory and so on.
Recently, there is an increasing demand of propolis. In the other hand the yield of
propolis is still relatively low. Such condition led to counterfeit of propolis. This
preliminary study tries to investigate the potential use of fluorescence spectroscopy for authentication of propolis product. Fluorescence spectra of eight propolis samples
including authentic and counterfeit propolis were recorded using excitation wavelength of 200 – 520 nm with 10 nm intervals and emission wavelength of 210 – 600 nm with 1 nm interval. From the analysis it is known that the counterfeit samples containing similar active compounds as the authentic one but in lower concentration. The obtained results indicated that fluorescence spectroscopy could be effectively used to differentiate the authentic and counterfeit propolis.

Keywords: Authentication, fluorescence spectroscopy, propolis.

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